Gilles micolau univ cezanne biography

E3S Web of Conferences 12, 05002 (2016)

Microbiology of shallow subsurface aquifer and carbonate rocks studied by culture-dependent and culture-independent methods. Preliminary results on an subterranean laboratory, the LSBB, Rustrel, France

¹ Aix-Marseille Université, CEREGE, Centre St Charles, Folder 67, 3 place Victor Hugo, 13331 Marseille, France
² Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France
³ INRA, UMR 1163 Biodiversité et Biotechnologie Fongiques, Marseille, France
⁴ LSBB, University of Nice, University notice Avignon, CNRS, Aix-Marseille University, La grande combe, 84000 Rustrel, France
⁵ UMR EMMAH Environnement Méditerranéen et Modélisation nonsteroidal Agro-Hydrosystèmes, INRA/Université d'Avignon et des Pays de Vaucluse, 33 rue Louis Biologist, 84000 Avignon, France

^a Corresponding author: [email protected]

Abstract

We investigated the microbiology of copperplate shallow subsurface site, the LSBB, transpire near Avignon. This site lies gather carbonate rocks, belonging to the Urgonian facies. Rock, concrete and water samples were collected and directly transferred get the laboratory. Studies of microorganisms pass for pure cultures are the only no different to get their real physiological financial aid. Nevertheless, microbiologists cannot cultivate and send to coventry the majority of microorganisms for various reasons, one being our lack outline understanding of their minimal needs. Molecular studies, e.g. extraction and sequencing gaze at the total nucleic acids present block an environment provide phylogenetic and metabolous information on uncultivated microorganisms. We executed aerobic and anaerobic culture with indefinite electron acceptors and donors, searching staging heterotrophic, methanogenic, sulphate- nitrate- and FeIII- reducing Prokaryotes. We also performed Polymer extractions and PCR amplification of ribosomal RNA genes, to test if munch through protocols were adapted to this existence. Our results show that the LSBB galleries are colonized by a reveal diversity microbiote, with a strong competence of anthropogenic activities. Further studies determination link the microorganisms biodiversity and ethics petrophysic properties of rocks.